To Develop a Simple (UV-VIS Spectrometric) Method for the Estimation of Multivitamin with Special Reference to Capsules & Tablets

Fat-soluble vitamin regulation is of particular significance in cystic fibrosis. A vitamin is an organic compound required as a nutrient in tiny amounts by an organism. In the present study, we had developed a simple method to estimate the fat soluble vitamin by Spectrophotometer. The vitamin A, D, E and K were estimated by spectrophotometer at 325 nm, 264 nm, 525 nm and 635 nm respectively. After calibration of the curves for the absorbance, the data were validated against label claimed on the packaging material. The precision, repeatability, intermediate precision and accuracy were tested. The accuracy of the method was determined by spiking working standards of the four vitamins into the placebo at different concentration levels: 80, 100 and 120% of target concentration of each of the vitamins. The mean recoveries (%) of vitamins A, D3, E and K3 were found to be 99.32, 98.68, 99.10 and 99.28 respectively which are within the acceptance limit. The spectrophotometer estimation is easier than other existing techniques in the pharmaceutical laboratories.